Deleted
Sample Discontinued
Experience: Works
Not Used
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Plasmid
psb1C3_int

Part:BBa_K743007:Design

Designed by:   Group: iGEM12_UC_Chile   (2012-09-20)


psb1C3_IntKR recombination plasmid for Synechocystis PCC6803


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

Using a double terminator followed by a reversed antibiotic resistance cds allows the use of the same terminator sequence for both the resistance casette and any desired coding sequence placed upstream


Source

Both BBa_K743001 and BBa_K743002 were PCR amplified from genomic dna. BBa_K743001 and BBa_B0015 were amplified from biobricks.

References